3509035
Promega T7 RNA Polymerase
DNA-dependent RNA polymerase for in vitro transcription.
Manufacturer: Fischer Scientific
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Color
Colorless
Purity
>90%
For Use With (Application)
Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage
-30°C to -10°C
Product Type
T7 RNA Polymerase
Form
Liquid
Components
5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
pH
7.7
Storage Buffer
20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Source
Recombinant E. coli strain
Inhibitors
Recombinant RNasin™ Ribonuclease
Related Products
Description
- DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences >90% pure as determined by SDS polyacrylamide gel electrophoresis Free of detectable levels of contaminating RNase and DNase activity ( Incorporates 32P, 33P, 3H and 35S nucleoside triphosphates
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Color: Colorless
Purity: >90%
For Use With (Application): Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage: -30°C to -10°C
Product Type: T7 RNA Polymerase
Form: Liquid
Components: 5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
pH: 7.7
Storage Buffer: 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Source: Recombinant E. coli strain
Inhibitors: Recombinant RNasin™ Ribonuclease
Color:
Colorless
Purity:
>90%
For Use With (Application):
Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage:
-30°C to -10°C
Product Type:
T7 RNA Polymerase
Form:
Liquid
Components:
5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
pH:
7.7
Storage Buffer:
20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Source:
Recombinant E. coli strain
Inhibitors:
Recombinant RNasin™ Ribonuclease
Color: Colorless
Purity: __
For Use With (Application): Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Content And Storage: -30°C to -10°C
Product Type: Terminal Deoxynucleotidyl Transferase
Form: Liquid
Components: 5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
pH: 6.4
Storage Buffer: 50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Source: Recombinant E. coli strain
Inhibitors: __
Color:
Colorless
Purity:
__
For Use With (Application):
Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Content And Storage:
-30°C to -10°C
Product Type:
Terminal Deoxynucleotidyl Transferase
Form:
Liquid
Components:
5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
pH:
6.4
Storage Buffer:
50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Source:
Recombinant E. coli strain
Inhibitors:
__
Color: Colorless
Purity: >90%
For Use With (Application): Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Content And Storage: -30°C to -10°C
Product Type: Exonuclease III
Form: Liquid
Components: 10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
pH: 7.5
Storage Buffer: 20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Source: Recombinant E. coli strain
Inhibitors: 50mM NaCl
Color:
Colorless
Purity:
>90%
For Use With (Application):
Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Content And Storage:
-30°C to -10°C
Product Type:
Exonuclease III
Form:
Liquid
Components:
10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
pH:
7.5
Storage Buffer:
20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Source:
Recombinant E. coli strain
Inhibitors:
50mM NaCl
Color: __
Purity: __
For Use With (Application): __
Content And Storage: The unopened product requires storage at both -80°C and -20°C. Consult the product insert for specific storage temperatures. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Product Type: __
Form: __
Components: __
pH: __
Storage Buffer: __
Source: __
Inhibitors: __
Color:
__
Purity:
__
For Use With (Application):
__
Content And Storage:
The unopened product requires storage at both -80°C and -20°C. Consult the product insert for specific storage temperatures. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Product Type:
__
Form:
__
Components:
__
pH:
__
Storage Buffer:
__
Source:
__
Inhibitors:
__