PR-M4103

Promega T4 Polynucleotide Kinase

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 PR-M4103-Each-of-1 In Stock ₹ 85,474.44

PR-M4103 - Each of 1

₹ 85,474.44

In Stock

Quantity

1

Base Price: ₹ 85,474.44

GST (18%): ₹ 15,385.399

Total Price: ₹ 1,00,859.839

Concentration

5 to 10 U/μL

Components

10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT

pH

7.5

Storage Buffer

20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol

Source

Recombinant E. coli strain

Product Type

T4 Polynucleotide Kinase

Inhibitors

Ammonium Ions and low levels of Phosphate Buffers

Color

Colorless

Purity

≥90%

For Use With (Application)

5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning

Content And Storage

-30°C to -10°C

Quantity

1000 U

Molecular Weight (g/mol)

132

Form

Liquid

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Description

  • Transfers the γ-Phosphate from ATP to the 5′ End of Nucleotides Can be inactivated by heating at 68°C for 10 minutes Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Labels the 5′ end of ssDNA, dsDNA and RNA molecules for use as probes or for sequencing or DNA-protein footprinting Supplied at a concentration of 5-10u/μL

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Show Difference

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Fischer Scientific

PR-M4103

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Concentration:
5 to 10 U/μL

Components:
10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT

pH:
7.5

Storage Buffer:
20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol

Source:
Recombinant E. coli strain

Product Type:
T4 Polynucleotide Kinase

Inhibitors:
Ammonium Ions and low levels of Phosphate Buffers

Color:
Colorless

Purity:
≥90%

For Use With (Application):
5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning

Content And Storage:
-30°C to -10°C

Quantity:
1000 U

Molecular Weight (g/mol):
132

Form:
Liquid

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PR-M4211

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Concentration:
5 to 10 U/μL

Components:
10X Reaction Buffer: 250mM Tris acetate (pH 7.7), 1M Potassium Acetate, 100mM Magnesium Acetate and 10mM DTT

pH:
6.5

Storage Buffer:
200mM Potassium Phosphate (pH 6.5 at 25°C), 2mM DTT, 50% Glycerol

Source:
Recombinant E. coli strain

Product Type:
T4 DNA Polymerase

Inhibitors:
__

Color:
Colorless

Purity:
>95%

For Use With (Application):
Flush 5' protruding ends with labeled or unlabeled dNTPs, blunt 3' overhangs, in-vitro mutagenesis

Content And Storage:
-30°C to -10°C

Quantity:
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Molecular Weight (g/mol):
112

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Liquid

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10X Reaction Buffer: Tris HCl 100mM (pH 7.5 at 25°C), EDTA 50mM, Sodium Acetate 2M

pH:
7.3

Storage Buffer:
10mM Tris HCl (pH 8), 200mM NaCl, 50% Glycerol

Source:
Recombinant E. coli strain

Product Type:
Ribonuclease

Inhibitors:
__

Color:
Colorless

Purity:
__

For Use With (Application):
Mapping, RNase protection assay, mismatch detection, selective removal of RNA from DNA preparations

Content And Storage:
-30°C to -10°C

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1000 U

Molecular Weight (g/mol):
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Liquid

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5 to 10 U/μL

Components:
10X Reaction Buffer: Tris HCl 100mM (pH 7.5 at 25°C), EDTA 50mM, Sodium Acetate 2M

pH:
7.3

Storage Buffer:
10mM Tris HCl (pH 8), 200mM NaCl, 50% Glycerol

Source:
Recombinant E. coli strain

Product Type:
Ribonuclease

Inhibitors:
__

Color:
Colorless

Purity:
__

For Use With (Application):
Mapping, RNase protection assay, mismatch detection, selective removal of RNA from DNA preparations

Content And Storage:
-30°C to -10°C

Quantity:
5000 U

Molecular Weight (g/mol):
27

Form:
Liquid