3509034
Promega T4 Polynucleotide Kinase
T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group.
Manufacturer: Fischer Scientific
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Concentration
5 to 10 U/μL
Components
10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
pH
7.5
Storage Buffer
20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Source
Recombinant E. coli strain
Molecular Weight (g/mol)
132
Form
Liquid
Color
Colorless
Purity
≥90%
For Use With (Application)
5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Content And Storage
-30°C to -10°C
Product Type
T4 Polynucleotide Kinase
Inhibitors
Ammonium Ions and low levels of Phosphate Buffers
Related Products
Description
- Transfers the γ-Phosphate from ATP to the 5′ End of Nucleotides Can be inactivated by heating at 68°C for 10 minutes Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications Labels the 5′ end of ssDNA, dsDNA and RNA molecules for use as probes or for sequencing or DNA-protein footprinting Supplied at a concentration of 5-10u/μL
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Concentration: 5 to 10 U/μL
Components: 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
pH: 7.5
Storage Buffer: 20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Source: Recombinant E. coli strain
Molecular Weight (g/mol): 132
Form: Liquid
Color: Colorless
Purity: ≥90%
For Use With (Application): 5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Content And Storage: -30°C to -10°C
Product Type: T4 Polynucleotide Kinase
Inhibitors: Ammonium Ions and low levels of Phosphate Buffers
Concentration:
5 to 10 U/μL
Components:
10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
pH:
7.5
Storage Buffer:
20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Source:
Recombinant E. coli strain
Molecular Weight (g/mol):
132
Form:
Liquid
Color:
Colorless
Purity:
≥90%
For Use With (Application):
5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Content And Storage:
-30°C to -10°C
Product Type:
T4 Polynucleotide Kinase
Inhibitors:
Ammonium Ions and low levels of Phosphate Buffers
Concentration: __
Components: 5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
pH: 7.7
Storage Buffer: 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Source: Recombinant E. coli strain
Molecular Weight (g/mol): __
Form: Liquid
Color: Colorless
Purity: >90%
For Use With (Application): Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage: -30°C to -10°C
Product Type: T7 RNA Polymerase
Inhibitors: Recombinant RNasin™ Ribonuclease
Concentration:
__
Components:
5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
pH:
7.7
Storage Buffer:
20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Source:
Recombinant E. coli strain
Molecular Weight (g/mol):
__
Form:
Liquid
Color:
Colorless
Purity:
>90%
For Use With (Application):
Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage:
-30°C to -10°C
Product Type:
T7 RNA Polymerase
Inhibitors:
Recombinant RNasin™ Ribonuclease
Concentration: 30 U/μL
Components: 5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
pH: 6.4
Storage Buffer: 50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Source: Recombinant E. coli strain
Molecular Weight (g/mol): __
Form: Liquid
Color: Colorless
Purity: __
For Use With (Application): Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Content And Storage: -30°C to -10°C
Product Type: Terminal Deoxynucleotidyl Transferase
Inhibitors: __
Concentration:
30 U/μL
Components:
5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
pH:
6.4
Storage Buffer:
50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Source:
Recombinant E. coli strain
Molecular Weight (g/mol):
__
Form:
Liquid
Color:
Colorless
Purity:
__
For Use With (Application):
Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Content And Storage:
-30°C to -10°C
Product Type:
Terminal Deoxynucleotidyl Transferase
Inhibitors:
__
Concentration: 150 to 200 U/μL
Components: 10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
pH: 7.5
Storage Buffer: 20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Source: Recombinant E. coli strain
Molecular Weight (g/mol): 28000
Form: Liquid
Color: Colorless
Purity: >90%
For Use With (Application): Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Content And Storage: -30°C to -10°C
Product Type: Exonuclease III
Inhibitors: 50mM NaCl
Concentration:
150 to 200 U/μL
Components:
10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
pH:
7.5
Storage Buffer:
20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Source:
Recombinant E. coli strain
Molecular Weight (g/mol):
28000
Form:
Liquid
Color:
Colorless
Purity:
>90%
For Use With (Application):
Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Content And Storage:
-30°C to -10°C
Product Type:
Exonuclease III
Inhibitors:
50mM NaCl