PR-M2201
Promega Klenow Fragment, DNA Polymerase I
Manufacturer: Promega
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PR-M2201-Each-of-1 | In Stock | ₹ 23,101.20 |
PR-M2201 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 23,101.20
GST (18%): ₹ 4,158.216
Total Price: ₹ 27,259.416
Concentration
5 U/μL
Components
10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT
pH
7.2
Storage Buffer
10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source
Recombinant E. coli strain
Product Type
DNA Polymerase I Large (Klenow) Fragment
Form
Liquid
Color
Colorless
Purity
>80%
For Use With (Application)
Filling-in or labeling 3' ends of 5' overhanging double-stranded DNA, second-strand cDNA synthesis, DNA sequencing by the Dideoxy method
Content And Storage
-30°C to -10°C
Quantity
150 U
Molecular Weight (g/mol)
68
Related Products
Description
- DNA-Dependent DNA Polymerase that Lacks the 5′→3′ Exonuclease Activity Use in a variety of molecular applications Heat-inactivate by heating at 75°C for 10 minutes Active in many Promega 1X restriction enzyme buffers Supplied at a concentration of 5u/μL
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Concentration: 5 U/μL
Components: 10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT
pH: 7.2
Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source: Recombinant E. coli strain
Product Type: DNA Polymerase I Large (Klenow) Fragment
Form: Liquid
Color: Colorless
Purity: >80%
For Use With (Application): Filling-in or labeling 3' ends of 5' overhanging double-stranded DNA, second-strand cDNA synthesis, DNA sequencing by the Dideoxy method
Content And Storage: -30°C to -10°C
Quantity: 150 U
Molecular Weight (g/mol): 68
Concentration:
5 U/μL
Components:
10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT
pH:
7.2
Storage Buffer:
10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source:
Recombinant E. coli strain
Product Type:
DNA Polymerase I Large (Klenow) Fragment
Form:
Liquid
Color:
Colorless
Purity:
>80%
For Use With (Application):
Filling-in or labeling 3' ends of 5' overhanging double-stranded DNA, second-strand cDNA synthesis, DNA sequencing by the Dideoxy method
Content And Storage:
-30°C to -10°C
Quantity:
150 U
Molecular Weight (g/mol):
68
Concentration: 20 U/μL
Components: 10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
pH: 8
Storage Buffer: 10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
Source: Calf intestinal mucosa
Product Type: Alkaline Phosphatase
Form: Liquid
Color: Colorless
Purity: __
For Use With (Application): Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
Content And Storage: -30°C to -10°C
Quantity: 1000 U
Molecular Weight (g/mol): __
Concentration:
20 U/μL
Components:
10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
pH:
8
Storage Buffer:
10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
Source:
Calf intestinal mucosa
Product Type:
Alkaline Phosphatase
Form:
Liquid
Color:
Colorless
Purity:
__
For Use With (Application):
Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
Content And Storage:
-30°C to -10°C
Quantity:
1000 U
Molecular Weight (g/mol):
__
Concentration: 1 to 5 μg/μL
Components: 4 Identical 18.9kDa subunits
pH: 7.4
Storage Buffer: 50mM Tris HCl (pH 7.4), 1mM EDTA, 1mM DTT, 0.2M NaCl, 50% Glycerol
Source: E. coli
Product Type: Single-stranded DNA Binding Protein
Form: Liquid
Color: Colorless
Purity: __
For Use With (Application): Studying DNA replication and recombination, DNA sequencing
Content And Storage: -30°C to -10°C
Quantity: 100 μg
Molecular Weight (g/mol): __
Concentration:
1 to 5 μg/μL
Components:
4 Identical 18.9kDa subunits
pH:
7.4
Storage Buffer:
50mM Tris HCl (pH 7.4), 1mM EDTA, 1mM DTT, 0.2M NaCl, 50% Glycerol
Source:
E. coli
Product Type:
Single-stranded DNA Binding Protein
Form:
Liquid
Color:
Colorless
Purity:
__
For Use With (Application):
Studying DNA replication and recombination, DNA sequencing
Content And Storage:
-30°C to -10°C
Quantity:
100 μg
Molecular Weight (g/mol):
__
Concentration: 5 to 10 U/μL
Components: 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
pH: 7.5
Storage Buffer: 20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Source: Recombinant E. coli strain
Product Type: T4 Polynucleotide Kinase
Form: Liquid
Color: Colorless
Purity: ≥90%
For Use With (Application): 5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Content And Storage: -30°C to -10°C
Quantity: 100 U
Molecular Weight (g/mol): 132
Concentration:
5 to 10 U/μL
Components:
10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
pH:
7.5
Storage Buffer:
20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Source:
Recombinant E. coli strain
Product Type:
T4 Polynucleotide Kinase
Form:
Liquid
Color:
Colorless
Purity:
≥90%
For Use With (Application):
5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Content And Storage:
-30°C to -10°C
Quantity:
100 U
Molecular Weight (g/mol):
132