3509032
Promega T4 DNA Ligase
For ligation of DNA ends.
Manufacturer: Fischer Scientific
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Color
Colorless
Purity
>90%
For Use With (Application)
Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Content And Storage
-30°C to -10°C
Product Type
T4 DNA Ligase
Form
Liquid
Components
10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP
pH
7.4
Storage Buffer
10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source
RecombinantE. colistrain
Molecular Weight (g/mol)
68
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Description
- Joins Two DNA Strands with Cohesive or Blunt Ends Use with 5′, 3′ or blunt-ended dsDNA (e.g., inserts and vectors) Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule
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Color: Colorless
Purity: >90%
For Use With (Application): Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Content And Storage: -30°C to -10°C
Product Type: T4 DNA Ligase
Form: Liquid
Components: 10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP
pH: 7.4
Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source: RecombinantE. colistrain
Molecular Weight (g/mol): 68
Color:
Colorless
Purity:
>90%
For Use With (Application):
Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Content And Storage:
-30°C to -10°C
Product Type:
T4 DNA Ligase
Form:
Liquid
Components:
10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP
pH:
7.4
Storage Buffer:
10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source:
RecombinantE. colistrain
Molecular Weight (g/mol):
68
Color: Colorless
Purity: ≥90%
For Use With (Application): 5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Content And Storage: -30°C to -10°C
Product Type: T4 Polynucleotide Kinase
Form: Liquid
Components: 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
pH: 7.5
Storage Buffer: 20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Source: Recombinant E. coli strain
Molecular Weight (g/mol): 132
Color:
Colorless
Purity:
≥90%
For Use With (Application):
5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Content And Storage:
-30°C to -10°C
Product Type:
T4 Polynucleotide Kinase
Form:
Liquid
Components:
10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
pH:
7.5
Storage Buffer:
20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Source:
Recombinant E. coli strain
Molecular Weight (g/mol):
132
Color: Colorless
Purity: >90%
For Use With (Application): Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage: -30°C to -10°C
Product Type: T7 RNA Polymerase
Form: Liquid
Components: 5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
pH: 7.7
Storage Buffer: 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Source: Recombinant E. coli strain
Molecular Weight (g/mol): __
Color:
Colorless
Purity:
>90%
For Use With (Application):
Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage:
-30°C to -10°C
Product Type:
T7 RNA Polymerase
Form:
Liquid
Components:
5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
pH:
7.7
Storage Buffer:
20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Source:
Recombinant E. coli strain
Molecular Weight (g/mol):
__
Color: Colorless
Purity: __
For Use With (Application): Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Content And Storage: -30°C to -10°C
Product Type: Terminal Deoxynucleotidyl Transferase
Form: Liquid
Components: 5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
pH: 6.4
Storage Buffer: 50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Source: Recombinant E. coli strain
Molecular Weight (g/mol): __
Color:
Colorless
Purity:
__
For Use With (Application):
Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Content And Storage:
-30°C to -10°C
Product Type:
Terminal Deoxynucleotidyl Transferase
Form:
Liquid
Components:
5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
pH:
6.4
Storage Buffer:
50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Source:
Recombinant E. coli strain
Molecular Weight (g/mol):
__