PR-M2051
Promega DNA Polymerase I
Manufacturer: Promega
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PR-M2051-Each-of-1 | In Stock | ₹ 26,865.84 |
PR-M2051 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 26,865.84
GST (18%): ₹ 4,835.851
Total Price: ₹ 31,701.691
Concentration
5 to 10 U/μL
Components
10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, DTT 1mM
pH
7.5
Storage Buffer
50mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source
Recombinant E. coli strain
Product Type
DNA Polymerase I
Color
Colorless
Purity
>90%
For Use With (Application)
Labeling of DNA to high specific radioactivity by nick translation, second-strand cDNA synthesis
Content And Storage
-30°C to -10°C
Quantity
500 U
Form
Liquid
Related Products
Description
- Catalyzes the Template-Directed Polymerization of Nucleotides into Duplex DNA in a 5′→3′ Direction Can be used in a variety of molecular applications Is inactivated by heating at 68°C for 10 minutes Supplied at a concentration of 5-10u/μL
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Concentration: 5 to 10 U/μL
Components: 10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, DTT 1mM
pH: 7.5
Storage Buffer: 50mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source: Recombinant E. coli strain
Product Type: DNA Polymerase I
Color: Colorless
Purity: >90%
For Use With (Application): Labeling of DNA to high specific radioactivity by nick translation, second-strand cDNA synthesis
Content And Storage: -30°C to -10°C
Quantity: 500 U
Form: Liquid
Concentration:
5 to 10 U/μL
Components:
10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, DTT 1mM
pH:
7.5
Storage Buffer:
50mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source:
Recombinant E. coli strain
Product Type:
DNA Polymerase I
Color:
Colorless
Purity:
>90%
For Use With (Application):
Labeling of DNA to high specific radioactivity by nick translation, second-strand cDNA synthesis
Content And Storage:
-30°C to -10°C
Quantity:
500 U
Form:
Liquid
Concentration: 5 U/μL
Components: 10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT
pH: 7.2
Storage Buffer: 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source: Recombinant E. coli strain
Product Type: DNA Polymerase I Large (Klenow) Fragment
Color: Colorless
Purity: >80%
For Use With (Application): Filling-in or labeling 3' ends of 5' overhanging double-stranded DNA, second-strand cDNA synthesis, DNA sequencing by the Dideoxy method
Content And Storage: -30°C to -10°C
Quantity: 150 U
Form: Liquid
Concentration:
5 U/μL
Components:
10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT
pH:
7.2
Storage Buffer:
10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Source:
Recombinant E. coli strain
Product Type:
DNA Polymerase I Large (Klenow) Fragment
Color:
Colorless
Purity:
>80%
For Use With (Application):
Filling-in or labeling 3' ends of 5' overhanging double-stranded DNA, second-strand cDNA synthesis, DNA sequencing by the Dideoxy method
Content And Storage:
-30°C to -10°C
Quantity:
150 U
Form:
Liquid
Concentration: 20 U/μL
Components: 10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
pH: 8
Storage Buffer: 10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
Source: Calf intestinal mucosa
Product Type: Alkaline Phosphatase
Color: Colorless
Purity: __
For Use With (Application): Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
Content And Storage: -30°C to -10°C
Quantity: 1000 U
Form: Liquid
Concentration:
20 U/μL
Components:
10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
pH:
8
Storage Buffer:
10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
Source:
Calf intestinal mucosa
Product Type:
Alkaline Phosphatase
Color:
Colorless
Purity:
__
For Use With (Application):
Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
Content And Storage:
-30°C to -10°C
Quantity:
1000 U
Form:
Liquid
Concentration: 1 to 5 μg/μL
Components: 4 Identical 18.9kDa subunits
pH: 7.4
Storage Buffer: 50mM Tris HCl (pH 7.4), 1mM EDTA, 1mM DTT, 0.2M NaCl, 50% Glycerol
Source: E. coli
Product Type: Single-stranded DNA Binding Protein
Color: Colorless
Purity: __
For Use With (Application): Studying DNA replication and recombination, DNA sequencing
Content And Storage: -30°C to -10°C
Quantity: 100 μg
Form: Liquid
Concentration:
1 to 5 μg/μL
Components:
4 Identical 18.9kDa subunits
pH:
7.4
Storage Buffer:
50mM Tris HCl (pH 7.4), 1mM EDTA, 1mM DTT, 0.2M NaCl, 50% Glycerol
Source:
E. coli
Product Type:
Single-stranded DNA Binding Protein
Color:
Colorless
Purity:
__
For Use With (Application):
Studying DNA replication and recombination, DNA sequencing
Content And Storage:
-30°C to -10°C
Quantity:
100 μg
Form:
Liquid