3509040
Promega Exonuclease III
Exonuclease III is a 3' → 5' exonuclease specific for dsDNA.
Manufacturer: Fischer Scientific
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Concentration
150 to 200 U/μL
Components
10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
pH
7.5
Storage Buffer
20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Source
Recombinant E. coli strain
Molecular Weight (g/mol)
28000
Form
Liquid
Color
Colorless
Purity
>90%
For Use With (Application)
Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Content And Storage
-30°C to -10°C
Product Type
Exonuclease III
Inhibitors
50mM NaCl
Related Products
Description
- A 3′→5′ Exonuclease Specific for Double-Stranded DNA Control deletion rate by varying incubation temperature Inactivate Exonuclease III by heating at 75°C for 10 minutes Supplied at a concentration of 150-200u/μL
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Concentration: 150 to 200 U/μL
Components: 10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
pH: 7.5
Storage Buffer: 20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Source: Recombinant E. coli strain
Molecular Weight (g/mol): 28000
Form: Liquid
Color: Colorless
Purity: >90%
For Use With (Application): Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Content And Storage: -30°C to -10°C
Product Type: Exonuclease III
Inhibitors: 50mM NaCl
Concentration:
150 to 200 U/μL
Components:
10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
pH:
7.5
Storage Buffer:
20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Source:
Recombinant E. coli strain
Molecular Weight (g/mol):
28000
Form:
Liquid
Color:
Colorless
Purity:
>90%
For Use With (Application):
Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Content And Storage:
-30°C to -10°C
Product Type:
Exonuclease III
Inhibitors:
50mM NaCl
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Content And Storage: The unopened product requires storage at both -80°C and -20°C. Consult the product insert for specific storage temperatures. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
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Content And Storage:
The unopened product requires storage at both -80°C and -20°C. Consult the product insert for specific storage temperatures. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
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