PR-M1804

Promega T4 DNA Ligase

Manufacturer: Fischer Scientific

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Pack Size SKU Availability Price
Each of 1 PR-M1804-Each-of-1 In Stock ₹ 41,325.48

PR-M1804 - Each of 1

₹ 41,325.48

In Stock

Quantity

1

Base Price: ₹ 41,325.48

GST (18%): ₹ 7,438.586

Total Price: ₹ 48,764.066

Concentration

1 to 3 U/μL

Components

10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP

pH

7.4

Storage Buffer

10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol

Source

RecombinantE. colistrain

Product Type

T4 DNA Ligase

Form

Liquid

Color

Colorless

Purity

>90%

For Use With (Application)

Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)

Content And Storage

-30°C to -10°C

Quantity

500 U

Molecular Weight (g/mol)

68

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Description

  • Joins Two DNA Strands with Cohesive or Blunt Ends Use with 5′, 3′ or blunt-ended dsDNA (e.g., inserts and vectors) Qualified for blue/white cloning, providing a higher level of quality control for enzymes used in cloning applications Can catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule

Compare Similar Items

Show Difference

Img

Fischer Scientific

PR-M1804

--


Concentration:
1 to 3 U/μL

Components:
10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP

pH:
7.4

Storage Buffer:
10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol

Source:
RecombinantE. colistrain

Product Type:
T4 DNA Ligase

Form:
Liquid

Color:
Colorless

Purity:
>90%

For Use With (Application):
Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)

Content And Storage:
-30°C to -10°C

Quantity:
500 U

Molecular Weight (g/mol):
68

Img

Fischer Scientific

PR-M1811

--


Concentration:
150 to 200 U/μL

Components:
10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2

pH:
7.5

Storage Buffer:
20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol

Source:
Recombinant E. coli strain

Product Type:
Exonuclease III

Form:
Liquid

Color:
Colorless

Purity:
>90%

For Use With (Application):
Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA

Content And Storage:
-30°C to -10°C

Quantity:
5000 U

Molecular Weight (g/mol):
28000

Img

Fischer Scientific

PR-M1815

--


Concentration:
150 to 200 U/μL

Components:
10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2

pH:
7.5

Storage Buffer:
20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol

Source:
Recombinant E. coli strain

Product Type:
Exonuclease III

Form:
Liquid

Color:
Colorless

Purity:
>90%

For Use With (Application):
Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA

Content And Storage:
-30°C to -10°C

Quantity:
25,000 U

Molecular Weight (g/mol):
28000

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Fischer Scientific

PR-M1821

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Concentration:
1 U/μL

Components:
10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine

pH:
8

Storage Buffer:
10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol

Source:
Calf intestinal mucosa

Product Type:
Alkaline Phosphatase

Form:
Liquid

Color:
Colorless

Purity:
__

For Use With (Application):
Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase

Content And Storage:
-30°C to -10°C

Quantity:
1000 U

Molecular Weight (g/mol):
__