PR-M1815
Promega Exonuclease III
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PR-M1815-Each-of-1 | In Stock | ₹ 52,599.00 |
PR-M1815 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 52,599.00
GST (18%): ₹ 9,467.82
Total Price: ₹ 62,066.82
Concentration
150 to 200 U/μL
Components
10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
pH
7.5
Storage Buffer
20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Source
Recombinant E. coli strain
Product Type
Exonuclease III
Inhibitors
50mM NaCl
Color
Colorless
Purity
>90%
For Use With (Application)
Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Content And Storage
-30°C to -10°C
Quantity
25,000 U
Molecular Weight (g/mol)
28000
Form
Liquid
Related Products
Description
- A 3′→5′ Exonuclease Specific for Double-Stranded DNA Control deletion rate by varying incubation temperature Inactivate Exonuclease III by heating at 75°C for 10 minutes Supplied at a concentration of 150-200u/μL
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Concentration: 150 to 200 U/μL
Components: 10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
pH: 7.5
Storage Buffer: 20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Source: Recombinant E. coli strain
Product Type: Exonuclease III
Inhibitors: 50mM NaCl
Color: Colorless
Purity: >90%
For Use With (Application): Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Content And Storage: -30°C to -10°C
Quantity: 25,000 U
Molecular Weight (g/mol): 28000
Form: Liquid
Concentration:
150 to 200 U/μL
Components:
10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
pH:
7.5
Storage Buffer:
20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Source:
Recombinant E. coli strain
Product Type:
Exonuclease III
Inhibitors:
50mM NaCl
Color:
Colorless
Purity:
>90%
For Use With (Application):
Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Content And Storage:
-30°C to -10°C
Quantity:
25,000 U
Molecular Weight (g/mol):
28000
Form:
Liquid
Concentration: 1 U/μL
Components: 10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
pH: 8
Storage Buffer: 10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
Source: Calf intestinal mucosa
Product Type: Alkaline Phosphatase
Inhibitors: __
Color: Colorless
Purity: __
For Use With (Application): Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
Content And Storage: -30°C to -10°C
Quantity: 1000 U
Molecular Weight (g/mol): __
Form: Liquid
Concentration:
1 U/μL
Components:
10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
pH:
8
Storage Buffer:
10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
Source:
Calf intestinal mucosa
Product Type:
Alkaline Phosphatase
Inhibitors:
__
Color:
Colorless
Purity:
__
For Use With (Application):
Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
Content And Storage:
-30°C to -10°C
Quantity:
1000 U
Molecular Weight (g/mol):
__
Form:
Liquid
Concentration: 30 U/μL
Components: 5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
pH: 6.4
Storage Buffer: 50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Source: Recombinant E. coli strain
Product Type: Terminal Deoxynucleotidyl Transferase
Inhibitors: __
Color: Colorless
Purity: __
For Use With (Application): Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Content And Storage: -30°C to -10°C
Quantity: 300 U
Molecular Weight (g/mol): __
Form: Liquid
Concentration:
30 U/μL
Components:
5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
pH:
6.4
Storage Buffer:
50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Source:
Recombinant E. coli strain
Product Type:
Terminal Deoxynucleotidyl Transferase
Inhibitors:
__
Color:
Colorless
Purity:
__
For Use With (Application):
Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Content And Storage:
-30°C to -10°C
Quantity:
300 U
Molecular Weight (g/mol):
__
Form:
Liquid
Concentration: 30 U/μL
Components: 5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
pH: 6.4
Storage Buffer: 50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Source: Recombinant E. coli strain
Product Type: Terminal Deoxynucleotidyl Transferase
Inhibitors: __
Color: Colorless
Purity: __
For Use With (Application): Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Content And Storage: -30°C to -10°C
Quantity: 1500 U
Molecular Weight (g/mol): __
Form: Liquid
Concentration:
30 U/μL
Components:
5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
pH:
6.4
Storage Buffer:
50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Source:
Recombinant E. coli strain
Product Type:
Terminal Deoxynucleotidyl Transferase
Inhibitors:
__
Color:
Colorless
Purity:
__
For Use With (Application):
Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Content And Storage:
-30°C to -10°C
Quantity:
1500 U
Molecular Weight (g/mol):
__
Form:
Liquid