PR-M2825

Promega Alkaline Phosphatase, Calf Intestinal

Manufacturer: Fischer Scientific

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Pack Size SKU Availability Price
Each of 1 PR-M2825-Each-of-1 In Stock ₹ 17,368.68

PR-M2825 - Each of 1

₹ 17,368.68

In Stock

Quantity

1

Base Price: ₹ 17,368.68

GST (18%): ₹ 3,126.362

Total Price: ₹ 20,495.042

Concentration

20 U/μL

Components

10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine

For Use With (Application)

Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase

Content And Storage

-30°C to -10°C

Quantity

1000 U

Form

Liquid

Color

Colorless

pH

8

Storage Buffer

10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol

Source

Calf intestinal mucosa

Product Type

Alkaline Phosphatase

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Description

  • Catalyzes the Hydrolysis of 5′ Phosphate Groups from DNA, RNA, and r- and dNTPs Available at high concentration; Cat.# M2825 contains 1,000 units of CIAP at 20u/μL Qualified for blue/white cloning; our assay provides a higher level of quality control for enzymes used in cloning applications

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Fischer Scientific

PR-M2825

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Concentration:
20 U/μL

Components:
10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine

For Use With (Application):
Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase

Content And Storage:
-30°C to -10°C

Quantity:
1000 U

Form:
Liquid

Color:
Colorless

pH:
8

Storage Buffer:
10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol

Source:
Calf intestinal mucosa

Product Type:
Alkaline Phosphatase

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Fischer Scientific

PR-M3011

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Concentration:
1 to 5 μg/μL

Components:
4 Identical 18.9kDa subunits

For Use With (Application):
Studying DNA replication and recombination, DNA sequencing

Content And Storage:
-30°C to -10°C

Quantity:
100 μg

Form:
Liquid

Color:
Colorless

pH:
7.4

Storage Buffer:
50mM Tris HCl (pH 7.4), 1mM EDTA, 1mM DTT, 0.2M NaCl, 50% Glycerol

Source:
E. coli

Product Type:
Single-stranded DNA Binding Protein

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PR-M4101

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Concentration:
5 to 10 U/μL

Components:
10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT

For Use With (Application):
5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning

Content And Storage:
-30°C to -10°C

Quantity:
100 U

Form:
Liquid

Color:
Colorless

pH:
7.5

Storage Buffer:
20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol

Source:
Recombinant E. coli strain

Product Type:
T4 Polynucleotide Kinase

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PR-M4103

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Concentration:
5 to 10 U/μL

Components:
10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT

For Use With (Application):
5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning

Content And Storage:
-30°C to -10°C

Quantity:
1000 U

Form:
Liquid

Color:
Colorless

pH:
7.5

Storage Buffer:
20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol

Source:
Recombinant E. coli strain

Product Type:
T4 Polynucleotide Kinase