3508996
Promega Alkaline Phosphatase, Calf Intestinal
Alkaline Phosphatase catalyzes the hydrolysis of 5'-phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates.
Manufacturer: Fischer Scientific
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Color
Colorless
pH
8
Storage Buffer
10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
Source
Calf intestinal mucosa
Product Type
Alkaline Phosphatase
Components
10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
For Use With (Application)
Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
Content And Storage
-30°C to -10°C
Quantity
1000 U
Form
Liquid
Related Products
Description
- Catalyzes the Hydrolysis of 5′ Phosphate Groups from DNA, RNA, and r- and dNTPs Available at high concentration; Cat.# M2825 contains 1,000 units of CIAP at 20u/μL Qualified for blue/white cloning; our assay provides a higher level of quality control for enzymes used in cloning applications
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Color: Colorless
pH: 8
Storage Buffer: 10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
Source: Calf intestinal mucosa
Product Type: Alkaline Phosphatase
Components: 10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
For Use With (Application): Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
Content And Storage: -30°C to -10°C
Quantity: 1000 U
Form: Liquid
Color:
Colorless
pH:
8
Storage Buffer:
10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
Source:
Calf intestinal mucosa
Product Type:
Alkaline Phosphatase
Components:
10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
For Use With (Application):
Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
Content And Storage:
-30°C to -10°C
Quantity:
1000 U
Form:
Liquid
Color: Colorless
pH: 7.8
Storage Buffer: 20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 0.2mg/mL BSA, 50% Glycerol
Source: Recombinant E. coli strain
Product Type: Ribonuclease H
Components: __
For Use With (Application): Removal of the RNA strand prior to second strand cDNA synthesis, analysis of in-vitro polyadenylation reaction products, microarrays
Content And Storage: -30°C to -10°C
Quantity: __
Form: Liquid
Color:
Colorless
pH:
7.8
Storage Buffer:
20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 0.2mg/mL BSA, 50% Glycerol
Source:
Recombinant E. coli strain
Product Type:
Ribonuclease H
Components:
__
For Use With (Application):
Removal of the RNA strand prior to second strand cDNA synthesis, analysis of in-vitro polyadenylation reaction products, microarrays
Content And Storage:
-30°C to -10°C
Quantity:
__
Form:
Liquid
Color: Colorless
pH: 7.3
Storage Buffer: 10mM Tris HCl (pH 8), 200mM NaCl, 50% Glycerol
Source: Recombinant E. coli strain
Product Type: Ribonuclease
Components: 10X Reaction Buffer: Tris HCl 100mM (pH 7.5 at 25°C), EDTA 50mM, Sodium Acetate 2M
For Use With (Application): Mapping, RNase protection assay, mismatch detection, selective removal of RNA from DNA preparations
Content And Storage: -30°C to -10°C
Quantity: __
Form: Liquid
Color:
Colorless
pH:
7.3
Storage Buffer:
10mM Tris HCl (pH 8), 200mM NaCl, 50% Glycerol
Source:
Recombinant E. coli strain
Product Type:
Ribonuclease
Components:
10X Reaction Buffer: Tris HCl 100mM (pH 7.5 at 25°C), EDTA 50mM, Sodium Acetate 2M
For Use With (Application):
Mapping, RNase protection assay, mismatch detection, selective removal of RNA from DNA preparations
Content And Storage:
-30°C to -10°C
Quantity:
__
Form:
Liquid
Color: Colorless
pH: 7.5
Storage Buffer: 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Source: Recombinant E. coli strain
Product Type: SP6 RNA Polymerase
Components: 100mM DTT 5X Reaction Buffer, transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
For Use With (Application): Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage: -30°C to -10°C
Quantity: __
Form: Liquid
Color:
Colorless
pH:
7.5
Storage Buffer:
20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Source:
Recombinant E. coli strain
Product Type:
SP6 RNA Polymerase
Components:
100mM DTT 5X Reaction Buffer, transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
For Use With (Application):
Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage:
-30°C to -10°C
Quantity:
__
Form:
Liquid