PR-M1821

Promega Alkaline Phosphatase, Calf Intestinal

Manufacturer: Fischer Scientific

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Pack Size SKU Availability Price
Each of 1 PR-M1821-Each-of-1 In Stock ₹ 16,684.20

PR-M1821 - Each of 1

₹ 16,684.20

In Stock

Quantity

1

Base Price: ₹ 16,684.20

GST (18%): ₹ 3,003.156

Total Price: ₹ 19,687.356

Concentration

1 U/μL

Components

10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine

For Use With (Application)

Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase

Content And Storage

-30°C to -10°C

Quantity

1000 U

Form

Liquid

Color

Colorless

pH

8

Storage Buffer

10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol

Source

Calf intestinal mucosa

Product Type

Alkaline Phosphatase

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Description

  • Catalyzes the Hydrolysis of 5′ Phosphate Groups from DNA, RNA, and r- and dNTPs Available at high concentration; Cat.# M2825 contains 1,000 units of CIAP at 20u/μL Qualified for blue/white cloning; our assay provides a higher level of quality control for enzymes used in cloning applications

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Fischer Scientific

PR-M1821

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Concentration:
1 U/μL

Components:
10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine

For Use With (Application):
Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase

Content And Storage:
-30°C to -10°C

Quantity:
1000 U

Form:
Liquid

Color:
Colorless

pH:
8

Storage Buffer:
10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol

Source:
Calf intestinal mucosa

Product Type:
Alkaline Phosphatase

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Concentration:
30 U/μL

Components:
5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT

For Use With (Application):
Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays

Content And Storage:
-30°C to -10°C

Quantity:
300 U

Form:
Liquid

Color:
Colorless

pH:
6.4

Storage Buffer:
50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol

Source:
Recombinant E. coli strain

Product Type:
Terminal Deoxynucleotidyl Transferase

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Concentration:
30 U/μL

Components:
5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT

For Use With (Application):
Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays

Content And Storage:
-30°C to -10°C

Quantity:
1500 U

Form:
Liquid

Color:
Colorless

pH:
6.4

Storage Buffer:
50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol

Source:
Recombinant E. coli strain

Product Type:
Terminal Deoxynucleotidyl Transferase

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