Thermo Scientific™ Klenow Fragment (10 U/μL)

Description

• Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I
• It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I
• Highlights Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides) Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers Applications DNA blunting by fill-in 3'-overhangs Random-primed DNA labeling Labeling by fill-in 5'-overhangs of dsDNA DNA sequencing by the Sanger method Site-specific mutagenesis of DNA with synthetic oligonucleotides Second strand synthesis of cDNA