4521356

Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)

Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.

Manufacturer: Fischer Scientific

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Concentration

5 U/μL

Polymerase

DNA Polymerase I

Compatible Buffer

Restriction Enzyme, PCR, RT Buffer

Product Type

Klenow Fragment

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Description

  • Description Klenow Fragment, exo-, is the large fragment of DNA polymerase I
  • It exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities of DNA Polymerase I
  • The 3'→5' exonuclease activity of the enzyme is eliminated by mutations in the 3'→5'-exonuclease active sit
  • Highlights Lacks 3'→5' exonuclease activity Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides) Active in restriction enzyme, PCR, and RT buffers

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