Thermo Scientific™ DNase I, RNase-free (1 U/μL)

Description

• Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA
• It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups
• The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ions
• In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides Highlights Recombinant enzyme Purified from non-animal host with a lower level of intrinsic RNases Applications Preparation of DNA-free RNA Removal of template DNA following in vitro transcription Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR DNA labeling by nick-translation in conjunction with DNA Polymerase I Studies of DNA-protein interactions by DNase I, RNase-free footprinting Generation of a library of randomly overlapping DNA inserts
• Reaction buffer containing Mn2+ is used Note DNase I is sensitive to physical denaturation
• Mix gently by inverting the tube
• Do not vortex.