4521378

Thermo Scientific™ RNase H (5 U/μL)

Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.

Manufacturer: Fischer Scientific

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Concentration

5 U/μL

Enzyme

RNase

Compatible Buffer

10X Reaction Buffer

Product Type

RNase H

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Description

  • Ribonuclease H (Rnase H) specifically degrades the RNA strand in RNA-DNA hybrids
  • It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA
  • Applications Removal of mRNA prior to synthesis of second strand cDNA RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT) Site-specific cleavage of RNA Studies of in vitro polyadenylation reaction products

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