Ribonuclease H (Rnase H) specifically degrades the RNA strand in RNA-DNA hybrids
It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA
Applications Removal of mRNA prior to synthesis of second strand cDNA RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT) Site-specific cleavage of RNA Studies of in vitro polyadenylation reaction products