Ribonuclease I (RNase I), an endoribonuclease, preferentially hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates via nucleoside 2', 3'-cyclic monophosphate intermediates
The enzyme does not require any metal ions for activity
Highlights Stable and active under a wide variety of reaction conditions Can be heat inactivated in 30 minutes at 100°C