Thermo Scientific™ T4 beta-glucosyltransferase

Description

• T4 β-glucosyltransferase (T4 BGT) transfers the glucose moiety of uridine diphosphoglucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA generating β-glucosyl-5 hydroxymethylcytosine
• Thermo Scientific T4 BGT is specifically formulated for fast reaction times without compromising the reaction efficiency
• The enzyme completes 5-hmC glucosylation of 1ωg DNA at 37°C in 15 minutes
• Highlights Specific – selectively transfers glucose to the hydroxymethyl moiety of 5-hmC Fast – complete glucosylation of 1μg DNA in just 15 min Convenient – supplied with optimized buffer and UDP-glucose Applications Locus specific detection of 5-hmC Enrichment of 5-hmC containing DNA Labeling of 5-hmC residues using a radioactive UDP-glucose donor Includes T4 β-glucosyltransferase, 5 U/μL 10X Epi buffer 10X UDP-glucose