Thermo Scientific™ CpG Methyltransferase (M.SssI)

Description

• The enzyme methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5'-CpG-3' context
• M.SssI is specifically formulated for fast reaction times without compromising the reaction efficiency
• The enzyme completes modification of all CpGs in 15 minutes at 37°C
• In addition, the enzyme has been specifically validated for use with genomic DNA -the primary substrate in epigenetic studies
• M.SssI is supplied with an optimized 10X reaction buffer and 50X S-adenosylmethionine (SAM) as a cofactor
• Highlights High efficiency -complete in vitro methylation of all CpGs in non-methylated and hemi-methylated DNA Fast -reaction is completed in 15 minutes at 37°C Stable -at -20°C for 2 years