Thermo Scientific™ EpiJET DNA Methylation Analysis Kit (MspI/HpaII)

Description

• 5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression
• EpiJET DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyze DNA methylation status at a specific locus
• Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation
• When the internal CpG in the 5'-CCGG-3' tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected
• The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour
• Highlights: • Fast —complete digestion of genomic DNA in 1 hour at 37°C • Efficient —specially formulated enzymes for epigenetic studies of DNA methylation status