Dryzyme™ Restriction Enzymes

Description

• Each enzyme catalyzes cleavage at or near the defined base sequence Utilized in cloning procedures Restriction endonucleases are purified Each lot produced meets quality control specifications Eco RI is isolated from E
• coli RY13 carrying RI (fi+ plasmid) genes for restriction and modification as well as genes for resistance to the drugs sulfathiazole and streptomycin
• The recognition specificity of Eco RI is sensitive to ionic environment
• 5'-GAATTC-3', breaks between GA 3'-CTTAAG-5', breaks between AG Hin d III —The first type restriction endonuclease activity was isolated from Haemophilus influenzae Rd cells by H
• O
• Smith and associates
• Subsequently, the presence of two enzymes ( Hin d II and Hin d III) in this cell strain was established
• 5'-AAGCTT-3', breaks between AA 3'-TTCGAA-5', breaks between AA Bam HI is isolated from Bacillus amyloliquefaciens H cells
• Under certain conditions, the enzyme will recognize variations of the palindrome sequence to include GGNNCC
• 5'-GGATCC-3', breaks between GG 3'-CCTAGG-5', breaks between GG One restriction enzyme unit is defined as the amount of enzyme required to digest 1.0μg of lambda phage DNA in 60 min
• at 37°C in a total reaction mixture of 50μL
• Order Info Products are lyophilized and contain 1500 units All enzyme shipments contain the appropriate reaction buffer for optimal reactions