4521398
Thermo Scientific™ BcuI (SpeI) (10 U/μL)
Cut at A^CTAGT sites with BcuI (SpeI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AhlI, SpeI).
Manufacturer: Fischer Scientific
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Concentration
10 U/μL
Enzyme
BcuI (SpeI)
Optimal Reaction Temperature
37°C
Product Type
Restriction Enzyme
Methylation Sensitivity
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer
10X Buffer Tango
Type IIS RE
No
Research Category
Traditional Cloning
Related Products
Description
- Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System
- In addition, the universal Tango buffer is provided for convenience in double digestions
- All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions
- To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations
- Highlights Superior quality -stringent quality control and industry leading manufacturing process Convenient color-coded Five Buffer System Includes universal Tango buffer for double-digestions BSA premixed in reaction buffers Wide selection of restriction endonuclease specificities
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Concentration: 10 U/μL
Enzyme: BcuI (SpeI)
Optimal Reaction Temperature: 37°C
Product Type: Restriction Enzyme
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Type IIS RE: No
Research Category: Traditional Cloning
Concentration:
10 U/μL
Enzyme:
BcuI (SpeI)
Optimal Reaction Temperature:
37°C
Product Type:
Restriction Enzyme
Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Type IIS RE:
No
Research Category:
Traditional Cloning
Concentration: 10 U/μL
Enzyme: Bpu1102I (BlpI)
Optimal Reaction Temperature: 37°C
Product Type: Restriction Enzyme
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Type IIS RE: No
Research Category: Traditional Cloning
Concentration:
10 U/μL
Enzyme:
Bpu1102I (BlpI)
Optimal Reaction Temperature:
37°C
Product Type:
Restriction Enzyme
Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Type IIS RE:
No
Research Category:
Traditional Cloning
Concentration: 5 U/μL
Enzyme: BplI
Optimal Reaction Temperature: 37°C
Product Type: Restriction Enzyme
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Type IIS RE: No
Research Category: Traditional Cloning
Concentration:
5 U/μL
Enzyme:
BplI
Optimal Reaction Temperature:
37°C
Product Type:
Restriction Enzyme
Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Type IIS RE:
No
Research Category:
Traditional Cloning
Concentration: 10 U/μL
Enzyme: Acc65I (Asp718I)
Optimal Reaction Temperature: 37°C
Product Type: Restriction Enzyme
Methylation Sensitivity: CpG Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dam Methylation-Sensitive
Compatible Buffer: 10X Buffer O
Type IIS RE: No
Research Category: Traditional Cloning
Concentration:
10 U/μL
Enzyme:
Acc65I (Asp718I)
Optimal Reaction Temperature:
37°C
Product Type:
Restriction Enzyme
Methylation Sensitivity:
CpG Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dam Methylation-Sensitive
Compatible Buffer:
10X Buffer O
Type IIS RE:
No
Research Category:
Traditional Cloning