Cut at GCCNNNN^NGGC sites with BglI restriction enzyme, which performs best at 37°C in O buffer.
Manufacturer: Fischer Scientific
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10 U/μL
Bgl I
Yes
No
Traditional Cloning
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
10X Buffer O
37°C
Restriction Enzyme