Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.
Manufacturer: Fischer Scientific
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2 U/μL
AarI
Yes
No
Traditional Cloning
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Unique Buffer (10X Buffer AarI)
37°C
Restriction Enzyme