4521791
Thermo Scientific™ HphI (10 U/μL)
The HphI restriction enzyme recognizes GGTGA(8/7)^ sites and cuts best at 37°C in B buffer (Isoschizomers: AsuHPI).
Manufacturer: Fischer Scientific
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Concentration
10 U/μL
Enzyme
HphI
Sensitive to Heat Inactivation
Yes
Type IIS RE
Yes
Research Category
Scarless Cloning
Methylation Sensitivity
Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer
10X Buffer B
Optimal Reaction Temperature
37°C
Product Type
Restriction Enzyme
Related Products
Description
- Lambda DNA ( dam- digested with HphI, 1.4% agarose, 168 cleavage sites conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System
- In addition, the universal Tango buffer is provided for convenience in double digestions
- All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions
- To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations
- Highlights Superior quality -stringent quality control and industry leading manufacturing process Convenient color-coded Five Buffer System Includes universal Tango buffer for double-digestions BSA premixed in reaction buffers Wide selection of restriction endonuclease specificities
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Concentration: 10 U/μL
Enzyme: HphI
Sensitive to Heat Inactivation: Yes
Type IIS RE: Yes
Research Category: Scarless Cloning
Methylation Sensitivity: Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X Buffer B
Optimal Reaction Temperature: 37°C
Product Type: Restriction Enzyme
Concentration:
10 U/μL
Enzyme:
HphI
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
Yes
Research Category:
Scarless Cloning
Methylation Sensitivity:
Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X Buffer B
Optimal Reaction Temperature:
37°C
Product Type:
Restriction Enzyme
Concentration: 10 U/μL
Enzyme: Hpa II
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Research Category: Traditional Cloning
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Optimal Reaction Temperature: 37°C
Product Type: Restriction Enzyme
Concentration:
10 U/μL
Enzyme:
Hpa II
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Research Category:
Traditional Cloning
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Optimal Reaction Temperature:
37°C
Product Type:
Restriction Enzyme
Concentration: 10 U/μL
Enzyme: Hinf I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Research Category: Traditional Cloning
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X Buffer R
Optimal Reaction Temperature: 37°C
Product Type: Restriction Enzyme
Concentration:
10 U/μL
Enzyme:
Hinf I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Research Category:
Traditional Cloning
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X Buffer R
Optimal Reaction Temperature:
37°C
Product Type:
Restriction Enzyme
Concentration: 10 U/μL
Enzyme: HincII (HindII)
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Research Category: Traditional Cloning
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Optimal Reaction Temperature: 37°C
Product Type: Restriction Enzyme
Concentration:
10 U/μL
Enzyme:
HincII (HindII)
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Research Category:
Traditional Cloning
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Optimal Reaction Temperature:
37°C
Product Type:
Restriction Enzyme