4521829

Thermo Scientific™ KspAI (HpaI) (10 U/μL)

The KspAI (HpaI) restriction enzyme recognizes GTT^AAC sites and cuts best at 37°C in B buffer (Isoschizomers: HpaI).

Manufacturer: Fischer Scientific

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Concentration

10 U/μL

Enzyme

KspAI (HpaI)

Sensitive to Heat Inactivation

Yes

Type IIS RE

No

Research Category

Traditional Cloning

Methylation Sensitivity

CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive

Compatible Buffer

10X Buffer B

Optimal Reaction Temperature

37°C

Product Type

Restriction Enzyme

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Description

  • Lambda DNA digested with KspAI (HpaI), 0.7% agarose, 14 cleavage sites conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System
  • In addition, the universal Tango buffer is provided for convenience in double digestions
  • All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions
  • To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations
  • Highlights Superior quality -stringent quality control and industry leading manufacturing process Convenient color-coded Five Buffer System Includes universal Tango buffer for double-digestions BSA premixed in reaction buffers Wide selection of restriction endonuclease specificities

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4521829

The KspAI (HpaI) restriction enzyme...


Concentration:
10 U/μL

Enzyme:
KspAI (HpaI)

Sensitive to Heat Inactivation:
Yes

Type IIS RE:
No

Research Category:
Traditional Cloning

Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive

Compatible Buffer:
10X Buffer B

Optimal Reaction Temperature:
37°C

Product Type:
Restriction Enzyme

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Type IIS RE:
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Traditional Cloning

Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive

Compatible Buffer:
10X Buffer R

Optimal Reaction Temperature:
37°C

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Restriction Enzyme

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Enzyme:
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Methylation Sensitivity:
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