Cut at ACGT^ sites with TaiI (MaeII) restriction enzyme, which performs best at 65°C in R buffer (Isoschizomers: HpyCH4IV, MaeII).
Manufacturer: Fischer Scientific
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10 U/μL
TaiI (MaeII)
No
No
Traditional Cloning
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
10X Buffer R
65°C
Restriction Enzyme