Cut at C^CTAGG sites with XmaJI (AvrII) restriction enzyme, which functions best at 37°C in Tango buffer (Isoschizomers: AspA2I, AvrII, BlnI).
Manufacturer: Fischer Scientific
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10 U/μL
XmaJI (AvrII)
Yes
No
Traditional Cloning
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
10X Buffer Tango
37°C
Restriction Enzyme