Thermo Scientific™ FastDigest DpnI

Description

• Lambda DNA digested with DpnI, 1.4% agarose, 22 cleavage sites FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers
• The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps
• DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer
• Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix
• For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels
• Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects
• Highlights 100% activity of all FastDigest enzymes in the universal buffer 100% buffer compatibility with downstream applications Complete digestion in 5-15 minutes Direct loading on gels No star activity 176 FastDigest enzymes available