To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations
Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation
restriction enzymes exhibit 100% of their certified activity in the recommended buffer
The following enzymes require unique buffers for optimal digestion: AarI AjiI BamHI BfuI Bpu10I BseXI Bsp143I Cfr9I Cfr10I Eam1105I Ecl136II Eco52I EcoRI KpnI Lsp1109I PacI PasI Ppu21I SacI ScaI SdaI SduI SgeI TaqI