FERER0523
Thermo Scientific™ KpnI, HC (50 U/μL)
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERER0523-Each-of-1 | In Stock | ₹ 37,133.04 |
FERER0523 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 37,133.04
GST (18%): ₹ 6,683.947
Total Price: ₹ 43,816.987
Concentration
50 U/μL
Enzyme
Kpn I
Sensitive to Heat Inactivation
Yes
Type IIS RE
No
Product Type
Restriction Enzyme
Methylation Sensitivity
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer
Unique Buffer (10X Buffer KpnI)
Optimal Reaction Temperature
37°C
Quantity
20,000 units
Research Category
Traditional Cloning
Related Products
Description
- Thermo Scientific™ KpnI is available as a FastDigest enzyme which is used for rapid DNA digestion
- More than 95% of the digested DNA fragments can be ligated and recut at a 5'-termini concentration of 0.02μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with KpnI
- 5'..G G T A C▵C...3' 3'..C▵C A T G G...5' Conditions for 100% Activity: 1X Buffer KpnI: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl 2 , 0.02% Triton X-100 and 0.1mg/mL BSA Incubate at 37°C Storage Buffer: KpnI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol Methylation Effects: Dam: never overlaps —no effect Dcm: may overlap —no effect CpG: may overlap —no effect EcoKI: never overlaps —no effect EcoBI: never overlaps —no effect Digestion of Agarose-embedded DNA: Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours Note: High glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.
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Concentration: 50 U/μL
Enzyme: Kpn I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: Unique Buffer (10X Buffer KpnI)
Optimal Reaction Temperature: 37°C
Quantity: 20,000 units
Research Category: Traditional Cloning
Concentration:
50 U/μL
Enzyme:
Kpn I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
Unique Buffer (10X Buffer KpnI)
Optimal Reaction Temperature:
37°C
Quantity:
20,000 units
Research Category:
Traditional Cloning
Concentration: 10 U/μL
Enzyme: Kpn2I (BspEI)
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Optimal Reaction Temperature: 55°C
Quantity: 500 U
Research Category: Traditional Cloning
Concentration:
10 U/μL
Enzyme:
Kpn2I (BspEI)
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Optimal Reaction Temperature:
55°C
Quantity:
500 U
Research Category:
Traditional Cloning
Concentration: 10 U/μL
Enzyme: Kpn2I (BspEI)
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Optimal Reaction Temperature: 55°C
Quantity: 2500 U
Research Category: Traditional Cloning
Concentration:
10 U/μL
Enzyme:
Kpn2I (BspEI)
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Optimal Reaction Temperature:
55°C
Quantity:
2500 U
Research Category:
Traditional Cloning
Concentration: 10 U/μL
Enzyme: MspI (HpaII)
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: 10X Buffer Tango
Optimal Reaction Temperature: 37°C
Quantity: 3000 U
Research Category: Traditional Cloning
Concentration:
10 U/μL
Enzyme:
MspI (HpaII)
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Methylation Sensitivity:
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
10X Buffer Tango
Optimal Reaction Temperature:
37°C
Quantity:
3000 U
Research Category:
Traditional Cloning