FERER0803
Thermo Scientific™ HinfI, HC (50 U/μL)
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERER0803-Each-of-1 | In Stock | ₹ 17,539.80 |
FERER0803 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,539.80
GST (18%): ₹ 3,157.164
Total Price: ₹ 20,696.964
Concentration
50 U/μL
Enzyme
Hinf I
Sensitive to Heat Inactivation
Yes
Type IIS RE
No
Product Type
Restriction Enzyme
Methylation Sensitivity
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer
10X Buffer R
Optimal Reaction Temperature
37°C
Quantity
10,000 U
Research Category
Traditional Cloning
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Description
- Thermo Scientific™ HinfI is available as a FastDigest enzyme which is used for rapid DNA digestion
- More than 95% of the digested DNA fragments can be ligated and recut at a 5'-termini concentration of 1.3μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with HinfI
- 5'...G▵A N T C...3' 3'...C T N A▵G...5' Conditions for 100% Activity: 1X Buffer R: 10mM Tris-HCl (pH8.5 at 37°C), 10mM MgCl 2 , 100mM KCl and 0.1mg/mL BSA Incubate at 37°C Storage Buffer: HinfI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol Methylation Effects: Dam: never overlaps —no effect Dcm: never overlaps —no effect CpG: may overlap —cleavage impaired EcoKI: never overlaps —no effect EcoBI: may overlap —blocked Compatible Ends: G▴AWTC — PfeI
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Concentration: 50 U/μL
Enzyme: Hinf I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X Buffer R
Optimal Reaction Temperature: 37°C
Quantity: 10,000 U
Research Category: Traditional Cloning
Concentration:
50 U/μL
Enzyme:
Hinf I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X Buffer R
Optimal Reaction Temperature:
37°C
Quantity:
10,000 U
Research Category:
Traditional Cloning
Concentration: 10 U/μL
Enzyme: Mbo I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Methylation Sensitivity: Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X Buffer R
Optimal Reaction Temperature: 37°C
Quantity: 300 U
Research Category: Traditional Cloning
Concentration:
10 U/μL
Enzyme:
Mbo I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Methylation Sensitivity:
Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X Buffer R
Optimal Reaction Temperature:
37°C
Quantity:
300 U
Research Category:
Traditional Cloning
Concentration: 10 U/μL
Enzyme: Mbo I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Methylation Sensitivity: Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X Buffer R
Optimal Reaction Temperature: 37°C
Quantity: 1500 U
Research Category: Traditional Cloning
Concentration:
10 U/μL
Enzyme:
Mbo I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Methylation Sensitivity:
Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X Buffer R
Optimal Reaction Temperature:
37°C
Quantity:
1500 U
Research Category:
Traditional Cloning
Concentration: 5 U/μL
Enzyme: MboII
Sensitive to Heat Inactivation: Yes
Type IIS RE: Yes
Product Type: Restriction Enzyme
Methylation Sensitivity: Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X Buffer B
Optimal Reaction Temperature: 37°C
Quantity: 300 U
Research Category: Scarless Cloning
Concentration:
5 U/μL
Enzyme:
MboII
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
Yes
Product Type:
Restriction Enzyme
Methylation Sensitivity:
Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X Buffer B
Optimal Reaction Temperature:
37°C
Quantity:
300 U
Research Category:
Scarless Cloning