FERFD0593
Thermo Scientific™ FastDigest NotI
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERFD0593-Each-of-1 | In Stock | ₹ 4,192.44 |
FERFD0593 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 4,192.44
GST (18%): ₹ 754.639
Total Price: ₹ 4,947.079
Content And Storage
• FastDigest NotI (20 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme
Not I
Sensitive to Heat Inactivation
Yes
Type IIS RE
No
Product Type
Restriction Enzyme
Product Line
FastDigest™
Inactivation Temperature
80°C
Methylation Sensitivity
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature
37°C
Quantity
20 μL (20 Reactions)
Research Category
Traditional Cloning
Overhang
5' protruding ends
Related Products
Description
- FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers
- The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps
- DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer
- Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix
- For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels
- Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects
- Thermo Scientific FastDigest NotI restriction enzyme recognizes GC^GGCCGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer
- Isoschizomers: CciNI
- Thermo Scientific FastDigest NotI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers
- The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps
- See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes
- DNA modifying enzymes, such as Klenow Fragment (Cat
- No
- EP0051), T4 DNA Ligase (Cat
- No
- EL0014) , alkaline phosphatases and T4 DNA Polymerase (Cat
- No
- EP0061) all have 100% activity in FastDigest Buffer
- Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix
- For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels
- Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects
- Features • 100% activity of all FastDigest enzymes in the universal buffer • 100% buffer compatibility with downstream applications • Complete digestion in 5–15 minutes • Direct loading on gels • No star activity • 176 FastDigest enzymes available Applications • Molecular cloning • Restriction site mapping • Genotyping • Southern Blot • Restriction fragment length polymorphism (RFLP) • SNP analysis Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer
- For applications that require product analysis by fluorescence excitation (e.g
- concentration measurements in UV light) the colorless FastDigest Buffer is recommended
- For methylation sensitivity, refer to product specifications.
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Content And Storage: • FastDigest NotI (20 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme: Not I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Product Line: FastDigest™
Inactivation Temperature: 80°C
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature: 37°C
Quantity: 20 μL (20 Reactions)
Research Category: Traditional Cloning
Overhang: 5' protruding ends
Content And Storage:
• FastDigest NotI (20 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme:
Not I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Product Line:
FastDigest™
Inactivation Temperature:
80°C
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature:
37°C
Quantity:
20 μL (20 Reactions)
Research Category:
Traditional Cloning
Overhang:
5' protruding ends
Content And Storage: • FastDigest NotI (50 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme: Not I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Product Line: FastDigest™
Inactivation Temperature: 80°C
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature: 37°C
Quantity: 50 μL (50 Reactions)
Research Category: Traditional Cloning
Overhang: 5' protruding ends
Content And Storage:
• FastDigest NotI (50 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme:
Not I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Product Line:
FastDigest™
Inactivation Temperature:
80°C
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature:
37°C
Quantity:
50 μL (50 Reactions)
Research Category:
Traditional Cloning
Overhang:
5' protruding ends
Content And Storage: • FastDigest NotI (150 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme: Not I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Product Line: FastDigest™
Inactivation Temperature: 80°C
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature: 37°C
Quantity: 150 μL (150 Reactions)
Research Category: Traditional Cloning
Overhang: 5' protruding ends
Content And Storage:
• FastDigest NotI (150 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme:
Not I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Product Line:
FastDigest™
Inactivation Temperature:
80°C
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature:
37°C
Quantity:
150 μL (150 Reactions)
Research Category:
Traditional Cloning
Overhang:
5' protruding ends
Content And Storage: • FastDigest NotI (250 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme: Not I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Product Line: FastDigest™
Inactivation Temperature: 80°C
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature: 37°C
Quantity: 250 μL (250 Reactions)
Research Category: Traditional Cloning
Overhang: 5' protruding ends
Content And Storage:
• FastDigest NotI (250 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme:
Not I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Product Line:
FastDigest™
Inactivation Temperature:
80°C
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature:
37°C
Quantity:
250 μL (250 Reactions)
Research Category:
Traditional Cloning
Overhang:
5' protruding ends