FERFD0664
Thermo Scientific™ FastDigest SmaI
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERFD0664-Each-of-1 | In Stock | ₹ 7,255.49 |
FERFD0664 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 7,255.49
GST (18%): ₹ 1,305.988
Total Price: ₹ 8,561.478
Content And Storage
FastDigest SmaI (200 μL), 10X FastDigest Buffer (1.0 mL), 10X FastDigest Green Buffer (1.0 mL), Store at –20°C.
Enzyme
Sma I
Sensitive to Heat Inactivation
Yes
Type IIS RE
No
Product Type
Restriction Enzyme
Product Line
FastDigest™
Inactivation Temperature
65°C
Methylation Sensitivity
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature
37°C
Quantity
200 μL (200 Reactions)
Research Category
Traditional Cloning
Overhang
Blunt
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Description
- 5' CCC ↓ GGG 3' 3' GGG ↑ CCC 5' FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers
- The universal buffer allows rapid single-, double- or multiple-DNA digestion within 5-15 minutes, eliminating any need for buffer change or subsequent DNA clean-up steps
- DNA modifying enzymes, such as Klenow Fragment, T4 DNA ligase, alkaline phosphatases, and T4 DNA polymerase all have 100% activity in FastDigest Buffer
- Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix
- For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels
- Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects
- Features 100% activity of all FastDigest enzymes in the universal buffer 100% buffer compatibility with downstream applications Complete digestion in 5–15 minutes Direct loading on gels No star activity 176 FastDigest enzymes available Applications Molecular cloning Restriction site mapping Genotyping Southern Blot Restriction fragment length polymorphism (RFLP) SNP analysis Notes The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer
- For applications that require product analysis by fluorescence excitation (e.g., concentration measurements in UV light), the colorless FastDigest Buffer is recommended
- For methylation sensitivity, refer to product specifications.
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Content And Storage: FastDigest SmaI (200 μL), 10X FastDigest Buffer (1.0 mL), 10X FastDigest Green Buffer (1.0 mL), Store at –20°C.
Enzyme: Sma I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Product Line: FastDigest™
Inactivation Temperature: 65°C
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature: 37°C
Quantity: 200 μL (200 Reactions)
Research Category: Traditional Cloning
Overhang: Blunt
Content And Storage:
FastDigest SmaI (200 μL), 10X FastDigest Buffer (1.0 mL), 10X FastDigest Green Buffer (1.0 mL), Store at –20°C.
Enzyme:
Sma I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Product Line:
FastDigest™
Inactivation Temperature:
65°C
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature:
37°C
Quantity:
200 μL (200 Reactions)
Research Category:
Traditional Cloning
Overhang:
Blunt
Content And Storage: • FastDigest TaqI (400 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme: Taq I
Sensitive to Heat Inactivation: No
Type IIS RE: No
Product Type: Restriction Enzyme
Product Line: FastDigest™
Inactivation Temperature: __
Methylation Sensitivity: Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature: 65°C
Quantity: 400 μL (400 Reactions)
Research Category: Traditional Cloning
Overhang: 5' protruding ends
Content And Storage:
• FastDigest TaqI (400 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme:
Taq I
Sensitive to Heat Inactivation:
No
Type IIS RE:
No
Product Type:
Restriction Enzyme
Product Line:
FastDigest™
Inactivation Temperature:
__
Methylation Sensitivity:
Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature:
65°C
Quantity:
400 μL (400 Reactions)
Research Category:
Traditional Cloning
Overhang:
5' protruding ends
Content And Storage: • FastDigest XbaI (300 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme: Xba I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Product Line: FastDigest™
Inactivation Temperature: 65°C
Methylation Sensitivity: Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature: 37°C
Quantity: 300 μL (300 reactions)
Research Category: Traditional Cloning
Overhang: 5' protruding ends
Content And Storage:
• FastDigest XbaI (300 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme:
Xba I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Product Line:
FastDigest™
Inactivation Temperature:
65°C
Methylation Sensitivity:
Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature:
37°C
Quantity:
300 μL (300 reactions)
Research Category:
Traditional Cloning
Overhang:
5' protruding ends
Content And Storage: • FastDigest XbaI (750 μL)• 10X FastDigest Buffer (2 x 1.0 mL)• 10X FastDigest Green Buffer (2 x 1.0 mL)Store at –20°C.
Enzyme: Xba I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Product Line: FastDigest™
Inactivation Temperature: 65°C
Methylation Sensitivity: Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature: 37°C
Quantity: 750 μL (750 reactions)
Research Category: Traditional Cloning
Overhang: 5' protruding ends
Content And Storage:
• FastDigest XbaI (750 μL)• 10X FastDigest Buffer (2 x 1.0 mL)• 10X FastDigest Green Buffer (2 x 1.0 mL)Store at –20°C.
Enzyme:
Xba I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Product Line:
FastDigest™
Inactivation Temperature:
65°C
Methylation Sensitivity:
Dam Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature:
37°C
Quantity:
750 μL (750 reactions)
Research Category:
Traditional Cloning
Overhang:
5' protruding ends