FERFD1703
Thermo Scientific™ FastDigest DpnI
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERFD1703-Each-of-1 | In Stock | ₹ 4,064.10 |
FERFD1703 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 4,064.10
GST (18%): ₹ 731.538
Total Price: ₹ 4,795.638
Content And Storage
• FastDigest DpnI (50 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme
Dpn I
Sensitive to Heat Inactivation
Yes
Type IIS RE
No
Product Type
Restriction Enzyme
Product Line
FastDigest™
Inactivation Temperature
80°C
Methylation Sensitivity
Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature
37°C
Quantity
50 μL (50 Reactions)
Research Category
Traditional Cloning
Overhang
Blunt
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Description
- Lambda DNA digested with DpnI, 1.4% agarose, 22 cleavage sites FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers
- The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps
- DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer
- Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix
- For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels
- Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects
- Highlights 100% activity of all FastDigest enzymes in the universal buffer 100% buffer compatibility with downstream applications Complete digestion in 5-15 minutes Direct loading on gels No star activity 176 FastDigest enzymes available
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Content And Storage: • FastDigest DpnI (50 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme: Dpn I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Product Line: FastDigest™
Inactivation Temperature: 80°C
Methylation Sensitivity: Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature: 37°C
Quantity: 50 μL (50 Reactions)
Research Category: Traditional Cloning
Overhang: Blunt
Content And Storage:
• FastDigest DpnI (50 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme:
Dpn I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Product Line:
FastDigest™
Inactivation Temperature:
80°C
Methylation Sensitivity:
Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature:
37°C
Quantity:
50 μL (50 Reactions)
Research Category:
Traditional Cloning
Overhang:
Blunt
Content And Storage: • FastDigest DpnI (100 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme: Dpn I
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Product Line: FastDigest™
Inactivation Temperature: 80°C
Methylation Sensitivity: Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature: 37°C
Quantity: 100 μL (100 Reactions)
Research Category: Traditional Cloning
Overhang: Blunt
Content And Storage:
• FastDigest DpnI (100 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme:
Dpn I
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Product Line:
FastDigest™
Inactivation Temperature:
80°C
Methylation Sensitivity:
Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Compatible Buffer:
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature:
37°C
Quantity:
100 μL (100 Reactions)
Research Category:
Traditional Cloning
Overhang:
Blunt
Content And Storage: • FastDigest BseJI (200 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme: BseJI
Sensitive to Heat Inactivation: No
Type IIS RE: No
Product Type: Restriction Enzyme
Product Line: FastDigest™
Inactivation Temperature: __
Methylation Sensitivity: CpG Methylation-Sensitive, Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature: 65°C
Quantity: 200 μL (200 Reactions)
Research Category: Traditional Cloning
Overhang: Blunt
Content And Storage:
• FastDigest BseJI (200 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme:
BseJI
Sensitive to Heat Inactivation:
No
Type IIS RE:
No
Product Type:
Restriction Enzyme
Product Line:
FastDigest™
Inactivation Temperature:
__
Methylation Sensitivity:
CpG Methylation-Sensitive, Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature:
65°C
Quantity:
200 μL (200 Reactions)
Research Category:
Traditional Cloning
Overhang:
Blunt
Content And Storage: • FastDigest AasI (25 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme: AasI
Sensitive to Heat Inactivation: Yes
Type IIS RE: No
Product Type: Restriction Enzyme
Product Line: FastDigest™
Inactivation Temperature: 80°C
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature: 37°C
Quantity: 25 μL (25 Reactions)
Research Category: Traditional Cloning
Overhang: 3' protruding ends
Content And Storage:
• FastDigest AasI (25 μL)• 10X FastDigest Buffer (1.0 mL)• 10X FastDigest Green Buffer (1.0 mL)Store at –20°C.
Enzyme:
AasI
Sensitive to Heat Inactivation:
Yes
Type IIS RE:
No
Product Type:
Restriction Enzyme
Product Line:
FastDigest™
Inactivation Temperature:
80°C
Methylation Sensitivity:
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer:
10X FastDigest Buffer/FastDigest Green Buffer
Optimal Reaction Temperature:
37°C
Quantity:
25 μL (25 Reactions)
Research Category:
Traditional Cloning
Overhang:
3' protruding ends