Vivid™ CC Substrate

Description

• Vivid™ Substrates are blocked dyes that yield a minimal fluorescence signal until oxidation occurs
• Cytochrome P450 metabolism at either of two potential oxidation sites releases the highly fluorescent product
• They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes
• This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility
• When used in combination with CYP450 BACULOSOMES™ Plus Reagents as part of the Vivid™ CYP450 Screening Kits, the Vivid™ Substrates enable rapid, robust detection of cytochrome P450 inhibitors
• This particular substrate (Vivid ™ CC Substrate) yields a product that emits a blue fluorescence
• More About Vivid™ CYP450 Screening Kits Vivid™ CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition
• Vivid™ CYP450 Screening Kits include Vivid™ Substrate, Vivid™ Fluorescent Standard, reaction buffer, Vivid™ Regeneration System, NADP+, and CYP450 BACULOSOMES™ Plus Reagent
• CYP450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase
• CYP450 BACULOSOMES™ Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s
• >> View all Vivid™ CYP450 Screening Kits Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition For Research Use Only
• Not for any animal or human therapeutic or diagnostic use.