Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

Description

• General description: Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at >95% .The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
• Application: Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membraneConcentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganismsPurification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLCDesalting, buffer exchange, and protein dialysis
• Features and Benefits: Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%Concentrates 500 μL down to 15 μLConcentration factor 25x to 30xHigh sample recovery (greater than 90% of dilute starting solution)Fast processing time: 10 to 30 minutesReverse spin capability provides consistent recoveries
• Physical form: Color Code: Clear
• Legal Information: Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany