Thermo Scientific™ PRO Disk with Reagent

Description

• Identify yeast and anaerobic microorganisms, primarily Candida albicans and Clostridium difficile , using Thermo Scientific™ Remel™ PRO Kit
• PRO Kit is recommended for use in qualitative procedures for detection of the enzyme L-Proline aminopeptidase in yeast and bacterial cells
• The PRO Kit may be used as a single colony screening test to aid in the identification of Candida albicans and Clostridium difficile
• The PRO disk is 99% positive when testing C
• difficile from anaerobic cultures
• It is useful in differentiating other Gram-positive and Gram-negative bacilli
• Use PRO Kit for identification of Candida albicans and Clostridium difficile
• C
• albicans is the yeast pathogen most frequently isolated from clinical specimens 1
• In 1990, Perry et al
• evaluated a rapid test to aid in the identification of C
• albicans based on colorimetric detection of the enzyme, L-proline aminopeptidase 2
• C
• difficile is recognized as the major cause of antibiotic-associated pseudomembranous colitis and a frequent cause of hospital-acquired diarrhoea
• Fedorko and Williams reported detection of L-Proline aminopeptidase is useful for identification of C
• difficile because it is one of the few biochemical tests for which this organism is positive 3
• Easy to store - No special storage required Easy to read - After addition of one drop of Cinnamaldehyde a color change from red to pink color development indicates a positive test The PRO disk contains the substrate L-proline-β-naphthylamide
• This substrate is hydrolyzed by an aminopeptidase releasing β-naphthylamine
• After addition of Cinnamaldehyde Reagent, β-naphthylamine reacts with ρ- dimethyl-aminocinnamaldehyde to form a red-colored complex.