Thermo Scientific™ PathoDx™ Chlamydia Culture Confirmation Kit

Description

• Detect and identify Chlamydia by immunofluorescence technique after growth in tissue culture with Thermo Scientific™ Remel™ PathoDx™ Chlamydia Culture Confirmation Kit
• Chlamydiae are the most common etiologic agents in sexually transmitted diseases 1
• The genus Chlamydia is divided into three species: C
• trachomatis , C
• psittaci , and C
• pneumoniae , which share a common lipopolysaccharide antigen 2
• C
• trachomatis is responsible for serious and often asymptomatic infections in humans 3
• It is currently recognized as the cause of cervicitis and pelvic inflammatory disease (PID) in female 4,5,6
• The Chlamydia Culture Confirmation Kit uses fluorescein-conjugated murine monoclonal antibodies to stain inclusions and/or elementary bodies in cell monolayers 48 hours after inoculation of a clinical specimen
• The fluorescein-labeled monoclonal antibodies recognize both the major outer membrane protein (MOMP) of all C
• trachomatis serovars and the genus-specific lipopolysaccharide (LPS) of Chlamydia
• When the antibody conjugate is added to a cover slip containing ethanol-fixed tissue culture cells, the reagent binds specifically to any chlamydial inclusions present in the cells
• A washing step removes unbound antibody conjugate
• When the stained coverslip is viewed under a fluorescence microscope, large bright apple-green inclusions within infected cells are observed against a red counterstained background of cells
• Ready-to-use – Liquid reagents Convenient – Color coded dropper bottles Easy-to-interpret – Fixed, stained cultures that are positive for chlamydiae contain inclusion bodies that fluoresce bright green, against a cell monolayer counter-stained dark red
• The presence of one inclusion body is considered positive
• The Chlamydia Culture Confirmation Kit contains Chlamydia Confirmation Reagent and Mounting Fluid (R62230)
• Chlamydia Confirmation Reagent contains 5.5 mL of solution containing fluorescein-labeled purified murine monoclonal antibodies, Evans Blue counterstain, an inhibitor of nonspecific stain, and 0.098% sodium azide in a protein-stabilized buffer solution
• The Mounting Fluid provides 5.0 mL of mounting fluid, that contains of buffered glycerol, with 0.01% sodium azide as preservative
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.