Thermo Scientific™ Brilliance™ E. coli/Coliform Selective Medium (Dehydrated)

Description

• Detect and enumerate Escherichia coli and other coliforms in food and water samples with selective, chromogenic Thermo Scientific™ Brilliance ™ E
• coli / Coliform Selective Agar
• The agar base uses two chromogens to differentiate between Escherichia coli and other coliforms
• This results in purple E
• coli , as they are able to cleave both chromogens while other coliforms, giving pink colonies
• The recovery and enumeration of Escherichia coli and coliforms are important indicators of environmental and food hygiene
• Use Brilliance E
• coli / Coliform Selective Agar for detection and enumeration of Escherichia coli and other coliforms in food and water samples
• Easy-to-read : purple E
• coli colonies are easy to differentiate from pink colonies of other coliforms Selective : due to the presence of selective agent sodium lauryl sulphate the medium is selective against Gram-positive organisms
• Brilliance E
• coli / Coliform Selective Agar contains two chromogenic agents: Rose-Gal : detects ß-galactosidase activity X-Glu : detects ß-glucuronidase activity Detection of ß-glucuronidase activity is widely used to differentiate Escherichia coli , as this is present in E
• coli but not in other members of the coliform group
• As most coliforms are lactose-positive, ß-galactosidase activity, encoded by the lacZ gene, is then used to differentiate this group from other organisms able to grow on the selective medium
• This results in purple E
• coli , as they are able to cleave both chromogens with other coliforms, giving pink colonies, as they cleave only the galactoside chromogen
• The medium also contains sodium lauryl sulphate which acts as a selective agent, inhibiting the growth of Gram-positive organisms
• Due to the addition of tryptophan, the rapid confirmation of E
• coli by the formation of indole using a spot indole test is possible
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand