Thermo Scientific™ Kligler Iron Agar (Dehydrated)

Description

• Identify Enterobacteriaceae on the basis of double sugar fermentation and hydrogen sulphide production using Thermo Scientific™ Oxoid™ Kligler Iron Agar (Dehydrated)
• Klingler Iron Agar combines the features of Kligler's Lead Acetate medium 1,2,3 and Russell's Double Sugar Agar 4
• The medium is recommended for the identification of colonies picked off from plating media such as MacConkey Agar, Bismuth Sulphite Agar, or Desoxycholate Citrate Agar, etc
• Differentiate Enterobacteriaceae on the basis of double sugar fermentation and hydrogen sulphide production with Klinger Iron Agar
• Non lactose fermenters initially produce a yellow slant and butt as a result of glucose fermentation
• Once the glucose is depleted, the reactions reverts to red slants (alkaline) due to oxidation of acids
• Lactose fermenting organisms produce yellow slants and butts
• There is no reversion to red in the slant because enough acid is produced to maintain an acid pH under aerobic conditions
• Non-fermenters produce red slants and butts
• H 2 S production results in a blackening of the medium, either throughout the butt or in a ring formation near the top of the butt
• Gas production is demonstrated by the presence of bubbles or cracks in the medium
• Typical reactions observed on Kligler Iron Agar after 18-24 hours at 35 °C
• Organism Slope Butt Gas H 2 S Shigella sonnei Red Yellow - - Shigella dysenteriae Red Yellow - - Salmonella typhi Red Yellow - + Salmonella species Red Yellow + + Enterobacter species Red Yellow + - Klebsiella species Yellow Yellow + - Escherichia coli Yellow Yellow + - Proteus mirabilis Red Yellow - + Morganella species Red Yellow V - Citrobacter freundii Yellow Yellow + + Yersinia species Red Yellow V - Not all products are available for sale in all territories
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.