Thermo Scientific™ Staphylococcus Medium No. 110 (Dehydrated)

Description

• Selectively isolate and differentiate pathogenic staphylococci based on salt tolerance, pigmentation, mannitol fermentation and gelatin liquefaction with Thermo Scientific™ Oxoid™ Staphylococcus Medium No.110 (Dehydrated)
• Pathogenic staphylococci (coagulase-positive) grow on the high-salt mannitol medium to form orange colonies which give positive reactions for acid production and gelatin liquefaction
• Use Staphylococcus Medium No.110 for selective isolation and differentiation of pathogenic staphylococci (Chapman 1,2 ) based on salt tolerance, pigmentation, mannitol fermentation, and gelatin liquefaction
• The Staphylococcus Medium No.110 is formulated according to the APHA 3 and AOAC 4 specifications and can be used with Egg Yolk Emulsion (5% v/v SR0047) to study the egg yolk reactions
• Stone 5 suggested that the gelatinase activity in the medium was indicative of food poisoning strains but Chapman et al
• 6 reported that typical food poisoning staphylococci should also produce an orange pigment, be hemolytic, be coagulase-positive, and ferment mannitol
• Chapman 7 showed that incubation at 30°C produced deeper pigmentation and no interference with the Stone reaction or with acid production from mannitol - both latter being about as intense as at 35°C
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