Thermo Scientific™ EE Broth (Buffered Glucose Brilliant Green Bile Broth) (Dehydrated)

Description

• Enrich Enterobacteriaceae in bacteriological examination of foods 1 and animal feed 2 stuffs with Thermo Scientific™ Oxoid™ EE Broth (Buffered Glucose Brilliant Green Bile Broth) (Dehydrated)
• This medium is more inhibitory to non-Enterobacteriaceae than other non-selective media, e.g
• Mannitol broth 3 or Lactose broth 4 by virtue of the presence of brilliant green and bile salts in the preparation
• To monitor the sanitary quality of food and drugs, it is important to enumerate Enterobacteriaceae in samples accurately
• E.E
• Broth is recommended as an enrichment medium for Enterobacteriaceae in the bacteriological examination of foods and animal feedstuffs
• Selective : non- Enterobacteriaceae are inhibited due to brilliant green and bile salts in the medium Reliable : overcomes inhibition of small numbers of Enterobacteriaceae cells due to bile salt variations Accurate : the entire Enterobacteriaceae group can be used as indicator organisms avoiding discrepancies which can occur with standard `coli-aerogenes’ test The medium is more inhibitory to non- Enterobacteriaceae than other non-selective media (e.g., Mannitol broth or Lactose broth) due to the presence of brilliant green and bile salts
• The reliability of methods used to enumerate Enterobacteriaceae depends on resuscitation of damaged cells
• Weakened cells arise from exposure to dehydration, low pH and other unfavorable conditions
• E.E
• Broth’s formulation includes purified ox bile to overcome inhibition of small numbers of Enterobacteriaceae cells due to bile salt variations
• A preliminary assay can be used to check growth by inoculating approximately one viable cell per medium unit
• For the bacteriological evaluation of processed foods, the entire Enterobacteriaceae group can be used as indicator organisms
• This overcomes discrepancies that can arise when lactose-negative, anaerogenic lactose-positive or late lactose fermenting enterobacteria are present but are missed by the standard `coli-aerogenes’ tests
• To overcome these problems, lactose media have been replaced by those containing glucose
• Mossel et al 1 cited several examples which referred to foods contaminated with salmonellae, although results for coliforms were negative
• A later example quoted by Mossel 2 involved an outbreak of diarrhea caused by French mold-fermented soft cheese contaminated by Escherichia coli serotype O124
• This organism was lactose-negative and, therefore, was not detected in coliform tests, but only recognized when the commodity was tested for Enterobacteriaceae since it fermented glucose rapidly
• E.E
• Broth should be used as an enrichment broth in conjunction with Violet Red Bile Glucose Agar (Part No
• PO5043A)
• When specific organisms are required, rather than Enterobacteriaceae in general, subcultures must be made onto lactose differential media, e.g., Desoxycholate Citrate Agar
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand