Thermo Scientific™ Reinforced Clostridial Agar (RC Agar) (Dehydrated)

Description

• Cultivate and enumerate anaerobes, especially Clostridium species, with Thermo Scientific™ Oxoid™ Reinforced Clostridial Agar (RC Agar) (Dehydrated)
• A solid, highly nutritious medium, it can be used as an enrichment medium to optimize the growth of clostridia and other anaerobes, lactobacilli, and many other species of bacteria
• Use Reinforced Clostridial Agar for the isolation and cultivation of anaerobic organisms occurring in a variety of habitats, including food and pathological specimens
• Reinforced Clostridial Agar is a solid version of Thermo Scientific Oxoid Reinforced Clostridium Medium suitable for the cultivation and enumeration of clostridia and other anaerobes
• Reinforced Clostridial Agar does not differ significantly in performance from the pork-starch-pea agar of Anderson 1 for the count of anaerobes
• It is employed for the estimation of clostridia in food—see below (also Barnes et al
• 2 and Angelotti 3 )
• Attenborough and Scarr 4 employed RC Agar, in conjunction with membrane filters, for the count of Clostridium thermosaccharolyticum in sugar
• Reinforced Clostridial Agar is also frequently employed for the investigation of intestinal flora: Perry et al
• 5 for investigation of bovine rumen streptococci; Williams Smith & Crabb 6 used the Oxoid medium with added sodium chloride or blood for counts on human or animal feces; Barnes & Goldberg 7 employed the Oxoid medium with added chlortetracycline hydrochloride or sodium azide and ethyl violet, for the examination of poultry feces; the Oxoid medium was also used by Goldberg et al
• 8 for the examination of poultry fecal samples; Williams Smith 9 employed Oxoid Reinforced Clostridial Agar with added blood for the ‘total’ and Lactobacillus count of human and animal feces; with added blood and neomycin for determination of Bacteroides, anaerobic Gram-negative cocci, ‘total’ streptococci, sporeformers, yeasts, and ‘actino’ types
• Sneath 10 used Oxoid RC Agar and other media for the anaerobic count of microorganisms from soil samples up to approximately 300 years old
• Gregory et al
• 11 also employed the Oxoid medium for the estimation of anaerobes in molding hay
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