Thermo Scientific™ Baird-Parker Agar Base (Dehydrated)

Description

• Isolate and enumerate Staphylococcus aureus with selective and sensitive Thermo Scientific™ Oxoid™ Baird-Parker Agar Base (Dehydrated)
• Baird Parker Agar Base contains carefully balanced selective inhibitory agents, glycine, lithium, and tellurite, to suppress the growth of most of the bacteria present in foods without inhibiting coagulase-positive staphylococci
• The presence of egg yolk emulsion and sodium pyruvate aid the recovery of damaged cells for presumptive identification of Staphylococcus aureus in food specimens
• Baird-Parker Agar Base is widely recommended by national and international bodies for the isolation of Staphylococcus aureus 1
• Easy interpretation of test results as Staphylococcus aureus forms grey-black shiny colonies with surrounding clear zones
• Greater recovery of damaged staphylococci than on other media 2
• Baird-Parker 2 developed this medium from the tellurite-glycine formulation of Zebovitz et al
• 3 and improved its reliability in isolating Staphylococcus aureus from foods
• Baird-Parker added sodium pyruvate, to protect damaged cells and aid their recovery 3 Egg yolk emulsion makes the medium yellow and opaque
• Staphylococcus aureus reduces tellurite to form grey-black shiny colonies and then produces clear zones around the colonies by proteolytic action
• This clear zone with typical grey-black colony is diagnostic for Staphylococcus aureus
• On further incubation, most strains of Staphylococcus aureus form opaque haloes around the colonies
• Some strains of Staphylococcus saprophyticus may also produce both clear zones and opaque haloes but can be distinguished by the longer incubation required 4
• Colonies typical of Staphylococcus aureus, but without an egg yolk reaction, should also be tested for coagulase production 5
• Egg yolk reaction negative Staphylococcus aureus may occur in some food, especially cheese
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.