Thermo Scientific™ Iso-Sensitest Agar (Dehydrated)

Description

• Perform effortless antimicrobial susceptibility testing using Thermo Scientific™ Oxoid™ Iso-Sensitest Agar (Dehydrated), a semi-defined medium specifically designed for antimicrobial susceptibility testing (AST)
• Iso-Sensitest Broth is carefully constructed to give reproducible results
• It allows the growth of the majority of microorganisms without the need for further supplementation
• Iso-Sensitest Agar has been designed to overcome objections made about Mueller-Hinton Media by various workers 1,2,3,4,5,6,7 These objections were: Different MIC values in the broth and agar versions of the medium
• Agar versions showing antagonistic effects towards tetracycline
• High levels of sulphonamide and trimethoprim antagonists
• Poor reproducibility with different manufacturers' peptones
• Poor growth-supporting ability for streptococci and variable growth rates with gram-positive organisms generally Some mutant strains which are totally dependent on thymine and thymidine for their growth will, however, not grow in Iso-Sensitest Agar , which has these two compounds at very low levels, as they are the naturally occurring antagonists of trimethoprim
• Care must be taken to recognize these strains 8,9,10
• Iso-Sensitest Agar was developed from Oxoid™ Sensitest Agar (CM0409) which has been used successfully in several centers throughout the world 11,12,13
• The role of metal ions as antagonists to certain antibiotics has been described by many workers 14,15,16,17,18,19
• A stabilized mineral content in sensitivity test media is, therefore, important
• Reller, Schoenknecht, Kenny & Sherris 3 demonstrated the contribution of cations provided in media by ordinary agars
• A considerable difference in mineral content between the agar and broth media was shown
• Only by using an agar which is specially processed to remove the free anions and cations can a stabilized mineral content be obtained
• Iso-Sensitest Agar has stability in mineral content and the ability to produce optimum antimicrobial zones of inhibition
• The amino-nitrogen base of acid-hydrolyzed casein and special peptones has been supplemented with defined growth factors
• Careful preparation of the nutrients ensures that trimethoprim and sulphonamide antagonists are at very low levels
• Not all products are available for sale in all territories
• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.