Thermo Scientific™ Buffered Peptone Water (Dehydrated)

Description

• Pre-enrich food samples prior to the isolation of Salmonella species using Thermo Scientific™ Oxoid™ Buffered Peptone Water (Dehydrated)
• Buffered Peptone Water is a non-selective pre-enrichment medium
• It also provides conditions for resuscitation of cells that have been injured by processes of food preservation
• Low numbers and/or sub-lethally injured Salmonella spp
• cells may be present in a test food sample
• Pre-enrichment in non-selective Buffered Peptone Water allows the cells time to repair and multiply before being introduced into a selective culture, improving the chances of recovering Salmonella species from the sample
• Supports high growth and recovery rate : rich in nutrients, the medium produces high resuscitation rates and supports intense growth
• Helps recovery of sub-lethally damaged salmonellae : the phosphate buffer system of the medium prevents bacterial damage due to changes in pH of the medium and helps the recovery of sub-lethally damaged salmonellae that may be sensitive to low pH
• It was noted by Edel and Kampelmacher 1 that sub-lethal injury to salmonellae may occur in many food processes
• In a survey involving isolation of salmonellae from meat that had been artificially contaminated with sub-lethally injured organisms, pre-enrichment in buffered peptone water at 37°C for 18 hours before selection in Brilliant Green Tetrathionate Bile Broth showed superior results compared with a direct selection method
• Sadovski 2 reported that, in experiments involving isolation of salmonellae from frozen vegetables, the rapid drop in pH when using lactose broth 3 as a pre-enrichment medium was detrimental to the recovery of salmonellae
• This was due to the enhanced sensitivity to low pH of freeze-injured salmonellae which may contaminate frozen vegetables
• Pre-enrichment with Buffered Peptone Water maintained a high pH over a period of 24 hours of incubation
• The vegetable tissue has a low buffering capacity and the medium overcame this problem
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.