Thermo Scientific™ Campylobacter Blood-Free Selective Agar Base (Dehydrated)

Description

• Isolate Campylobacter jejuni , Campylobacter coli and Campylobacter laridis using Thermo Scientific™ Oxoid™ Campylobacter Blood-Free Selective Agar Base (Dehydrated) when used with CCDA Selective Supplement SR0155
• Campylobacter Blood-Free Selective Agar Base is based on the original formulation described by Bolton et.al 1 which was developed to replace blood with charcoal, ferrous sulphate and sodium pyruvate
• For improved selectivity cephazolin in the formulation was replaced by cefoperazone as a selective agent 2
• Campylobacter are Gram-negative, spirally shaped microaerophilic organisms which may be present in raw milk, untreated water, improperly handled food and undercooked meats, poultry and shellfish
• Human consumption of these organisms can result in a range of clinical illnesses from transient asymptomatic colonisation to severe dysentery
• The symptoms of Campylobacter enteritis include diarrhoea, stomach pain, nausea, fever, headache and muscle pain
• Use Campylobacter Blood-Free Selective Agar Base for isolation of Campylobacter species
• Selective: due to the presence of the selective agents like cefoperazone and amphotericin B the medium is selective for Campylobacter Suitable for isolation of Campylobacter spp from non-clinical samples following enrichment in Exeter broth 3
• Recommended by the U.K
• Ministry of Agriculture, Fisheries and Food (MAFF) as a validated method for isolation of Campylobacter from foods
• 4 The colonial morphology of campylobacters can be used as a guideline for identification to species level
• Campylobacter jejuni strains produce grey, moist flat spreading colonies
• Some strains may have a green hue or a dry appearance, with or without a metallic sheen
• Campylobacter coli strains tend to be creamy-grey in colour, moist, slightly raised and often produce discrete colonies
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• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand