Thermo Scientific™ Bacillus cereus Selective Agar Base (Dehydrated)

Description

• Selectively isolate and enumerate Bacillus cereus from food samples with Thermo Scientific™ Oxoid™ Bacillus cereus Selective Agar Base (Dehydrated)
• Bacillus cereus Selective Agar Base meets the requirements for a medium that is sufficiently selective to be able to detect small numbers of Bacillus cereus cells and spores in the presence of large numbers of other food contaminants
• The medium is also sufficiently diagnostic that colonies of Bacillus cereus are readily identified and confirmed by microscopic examination
• Bacillus cereus is a Gram-positive, rod-shaped beta hemolytic bacterium commonly found in soil and food
• The role of Bacillus cereus in food poisoning, particularly from the consumption of contaminated rice, is now well documented 1,2,3
• The organism has also been implicated in eye infections 4, 5 and a wide range of other conditions, including abscess formation, meningitis, septicemia and wound infection
• Bacillus cereus is recognized as a significant pathogen in post-operative and post-traumatic wounds of orthopedic patients 6
• Amongst veterinarians, Bacillus cereus is a known cause of disease, especially mastitis, in ewes and heifers 7
• Differential : differentiates cereus from other species of Bacillus based on their resistance to polymyxin, lack of mannitol fermentation, and the presence of lecithinase
• Selective : inhibits the growth of gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa due to the addition of polymixin B supplement
• Bacillus cereus Selective Agar is based on the highly specific diagnostic and selective PEMBA medium, developed by Holbrook and Anderson 1 for the isolation and enumeration of Bacillus cereus in foods
• It meets the requirements for a medium that is sufficiently selective to be able to detect small numbers of Bacillus cereus cells and spores in the presence of large numbers of other food contaminants
• The medium is also sufficiently diagnostic that colonies of Bacillus cereus are readily identified and confirmed by microscopic examination
• The primary diagnostic features of the medium are the colonial appearance, precipitation of hydrolyzed lecithin and the failure of Bacillus cereus to utilize mannitol
• The typical colonies of Bacillus cereus are crenated, about 5mm in diameter and have a distinctive turquoise to peacock blue color surrounded by a good egg yolk precipitate of the same color
• These features distinguish Bacillus cereus from other Bacillus spp
• except Bacillus thuringiensis
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand