Thermo Scientific™ Bolton Broth Base (Dehydrated)

Description

• Selectively pre-enrich Campylobacter in food samples with Thermo Scientific™ Oxoid™ Bolton Broth Base (Dehydrated)
• Avoid false-negative results due to sub-lethally injured cells with Bolton Broth Base as it increases the number of cells available for culture, first by resuscitating organisms and then by encouraging them to multiply
• Campylobacter are Gram-negative, spirally shaped microaerophilic organisms which may be present in raw milk, untreated water, improperly handled food and undercooked meats, poultry and shellfish
• Human consumption of these organisms can result in a range of clinical illnesses from transient asymptomatic colonization to severe dysentery
• The symptoms of Campylobacter enteritis include diarrhea, stomach pain, nausea, fever, headache and muscle pain
• Use Bolton Broth Base for better recovery of Campylobacter spp
• Developed to optimize the growth of Campylobacter from contaminated food and dairy products
• Developed to increase the recovery rate of Campylobacter with the addition of sodium metabisulphite and sodium pyruvate, which reduce toxic compounds and increase the recovery rate and aero-tolerance of the culture
• Bolton Selective Enrichment Broth contains nutrients to aid resuscitation of sublethally injured cells, and is formulated to avoid the need for a microaerobic atmosphere
• Initial incubation is carried out at 30°-37°C, depending on the type of food to be examined
• After the pre-enrichment, the incubation temperature is raised to 42°C to increase the selective pressures on competing organisms
• Inclusion of sodium metabisulphite and sodium pyruvate in Bolton Broth quenches toxic compounds that may form in the culture medium
• These additions also increase the aero-tolerance of the culture
• The antibiotics contained in Bolton Broth Selective Supplement SR0183 optimize selectivity for Campylobacter spp.: Vancomycin―active against Gram- positives; Cefoperazone ―predominantly active against Gram-negatives; Trimethoprim ―active against a wide variety of Gram-negative and Gram-positive organisms and  Cycloheximide ―active against yeasts
• Not all products are available for sale in all territories
• Please inquire.